Understanding the practical application of artificial intelligence (AI) in research is increasingly important as it becomes embedded in life sciences and bioinformatics. This paper reports on a multilingual survey, developed through community discussions at the 2025 BioHackathon in Japan and distributed through its networks, to capture current practices, successes, and challenges in AI adoption. The survey, offered in English, Japanese, and Thai, received 105 responses spanning diverse demographics, regions, and professional backgrounds. Findings reveal that most participants are frequent AI users, with tools like ChatGPT, Gemini, and Claude widely adopted, with ChatGPT as number one response. AI is primarily used to assist or draft tasks in coding, research, and writing, while full task automation remains uncommon, reflecting a preference for AI as a collaborative aid rather than a replacement. Successes were noted in efficiency, coding support, and proposal writing, whereas challenges centered on accuracy and reliability. Institutional support emerged as a key factor: respondents in Japan, Thailand, and the private sector reported stronger support and higher satisfaction than English-speaking or academic counterparts. By documenting real-world practices and concerns, this survey provides a valuable community-driven resource to guide responsible AI development and foster international collaboration in bioinformatics.