This preprint outlines the results of the “2nd SpatialData Hackathon” organised by the scverse and Bioconductor teams. The event gathered experts to advance spatial omics through four hackathon tracks: (i) R interoperability, (ii) accessibility and performance of visualization tools, (iii) design modernization for the SpatialData framework, and (iv) file formats and transformations (NGFF).Key achievements include extending the SpatialData, Zarr and bioimaging frameworks to R/Bioconductor ecosystem, improving visualization with 2.5D/3D rendering and a chunked multiscale point representation, introducing cloud-based IO and prototypes for lazy file linking and bidirectional element-table relationships, and developing a language-agnostic conformance test suite for OME-NGFF coordinate transformations. The hackathon fostered collaboration, creating infrastructure prototypes and identifying interoperability challenges. Documented on GitHub, these efforts brought together 24 participants from the US and Europe, promoting a FAIR ecosystem of spatial omics and imaging tools.